The extraction of Evans blue from plasma

The extraction of Evans blue (T1824) from plasma and the measurement of plasma volume.

Haemolysis and lipaemia interfere with the direct photometric estimation of Evans blue in plasma. During an investigation of blood volume changes in surgical shock using this dye, lipaemia was often encountered and it was necessary to use an extraction procedure for the estimation of the dye concentration. A rapid method was desired. The method of Crooke and Morris (1942) using a hydrochloric a...

The estimation of Evans blue in plasma.

An extraction technique for the estimation of Evans blue in plasma.

The dye-dilution method for the determination of plasma volume presents several problems, one of which is the estimation of dye concentration in plasma. It is with this problem that the present paper is primarily concerned. The most frequently used dye is Evans blue, T 1824. The colorimetric estimation of this dye in plasma is open to a number of possible errors, particularly those introduced b...

Quantitative extraction of Evans blue leaked from the vessels in the skin.

鈴木 守 ・新井 弘祐 Several investigators have used the extravasal leakage of the circulating dye as an indicator of the change in capillary permeability by measuring, for example, the mean diameter of the blueing (1-3), the visual grading of the blueing (4, 5) or size and inten sity of the staining (6-12). Studying the effects of organic solvents on the capillary permeability, one of the present autho...

Chromatographic determination of Evans blue in plasma and serum.

The methods at present available for the determination of the dye, Evans blue, in plasma have certain disadvantages. Direct colorimetry of dyed plasma is inapplicable where the plasma is haemolyzed, turbid or lipaemic. The butanol extraction procedure of Harington, Pochin & Squire (1940) is troublesome and time-consuming where many analyses must be carried out; its accuracy is lower than those ...